Darryl Gopaul, Leslie Hart, Brenda Cupp, Martin Lee
St. Joseph's Health Centre London, Ontario Canada and Great Smokies Diagnostic Laboratory, Asheville, North Carolina, USA
These are published studies conducted by some of our customers, Reprints are available on request.
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M. Valsorda, P. Lebel, J.-R. LaPointe, L. Kemp, C. Laferriére, «Hôpital Sainte-Justine» and «Université de Montréal»
The ISOPLATER is designed to automate and standardize the streaking of Petri dishes. We have evaluated the ISOPLATER in a large pediatric microbiology laboratory, comparing its automated streaking to the standard manual method.
Two hundred ten specimens were set up both by ISOPLATER and manual methods, using standardized inocula on paired plates incubated indentically: 99 urines (of which 15 were positive), 31 throat swabs, 19 vaginal secretions, 18 sputa, 18 stools, 18 pus, and 10 blood cultures. Incubation was done aerobically and anaerobically as needed by the specimen type.
No differences were observed on paired plates regarding: colony, morphology or numbers, spreading, isolated colonies, background flora, or contamination. Colony sizes were smaller and zones of hemolysis less well-defined on the plates streaked by the ISOPLATER when incubated in air, but no differences were observed when paired plates were incubated in CO2. Streaking delayed up to 30 minutes after inoculation did not change the final results of the culture, as evaluated in 12 urine and 8 throat cultures.
We conculde that the ISOPLATER gave results comparable to those of conventional manual streaking fora good variety of clinical specimens. The automatic streaker was easy to use, reliable and well-accepted by the technologists.